![]() ![]() The air gets circulated and filtered before it is blown to the workbench.īiological Safety Cabinets Class III (Image Source: )Ĭlass III biosafety cabinets are often termed as Glove Box. The open front draws the air by ensuring personal safety. Apart from the sterile work environment and operator protection it also provides product protection. There is a glass window between the user and the workbench that only allows the hands of the users to reach the workbench.īiological Safety Cabinets Class II (Image source:)Ĭlass II biosafety cabinets are also used when an operator works with low to medium risk biological organisms. In this cabinet air from outside is drawn inside via the front opening present in the cabinet, which then passes to the HEPA filter that eliminated the pathogens and discharges sterile air on the workbench. It provides operator protection along with a sterile work environment. They are: Biological Safety Cabinets Class I (Image Source:)Ĭlass I biosafety cabinets are used when an operator works with low to medium risk biological organisms. They too contain HEPA filters but there is one dissimilarity that Biosafety Cabinets offers complete protection to the users against harmful pathogens, since the contaminated air is filtered before it can come in contact with the users.īased on the risk factor there are three Biosafety Cabinet Designs available. Working with Biosafety Cabinetsīiosafety Cabinets are similar to the Laminar Air Flow cabinets. In these types of hood, the air blows across the workbench and it comes in contact with the user as well. In the Horizontal System, The HEPA filters are located on the rear side, at the back of the workbench. Horizontal Laminar Air Flow (Image Source: ) It is not blowing directly towards the operator and therefore, the operator also remains safe while the experiment runs under sterile conditions. The air blows on the workbench from the top and passes out from the holes present on the Hood’s base. In the Vertical System, the HEPA filter is located on the top of the workbench. They are: Vertical Laminar Air Flow (Image Source: ) Laminar Airflow is available in two designs based on the location of HEPA filters. This completely sterile air then flows on the workbench and allows contamination-free conduct of the experiment. The partially filtered air then passes through the HEPA filters that eliminate the micro-organisms and other smaller particles. Air is blown from the filter pad towards the fan, leaving behind all the particulate matters. 137-168)Ī Laminar Air Flow contains a High-Efficiency Particulates Air Filter (HEPA), along with a fan and a filter pad. “Chapter V Principles and Applications of Laminar-flow Devices.” Methods in microbiology. Laminar Air Flow: How the system works? Front View of Laminar Air Flow (Image Source: McDade, J. ![]() ![]() The continuous air blowing across the Laminar hood maintains sterility inside the hood that makes it an extremely suitable environment to work with cultures that can be only maintained under a clean and sterile environment. The unchanging direction is the key that avoids cross-contamination. Working with Laminar Air flowĪ Laminar Airflow, as defined in a previous Biomall article, is a sterile bench cabinet that passes filtered air through HEPA filters while maintaining a constant velocity in a particular direction. We can assure you that you will get all your answers by the time you end up reading this article.īefore we reach any conclusions let’s what is a biosafety cabinet and a laminar airflow and how both of them are different from each other. Are you wondering aseptic environment that suits you best for working with your experimental samples? Whether you should go for a Laminar Airflow or opt for a Biosafety cabinet? Whether it is your test samples you are looking for a sterile environment or you are working with an extremely contagious sample and concerned for your own safety as well? ![]()
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